H2S 2014参加登録受け付けています

On 2014/2/20 木曜日, in hypoxia reseacrh, by bodyhacker

H2S 2014

先日お知らせした

H2S 2014, the third international meeting on Hydrogen Sulfide Biology and its biomedical effects 

のabstractの受付は締め切りましたが参加登録は絶賛行われています。

ここからどうぞ


キム兄に「宣伝しろ」と云われた訳ではありません。念の為。


学生さんと行っていた実験ですが細胞が予定通りに「分化」せずノーカウントとなりました。  PMAをちゃんとはじめから調整すれば良かったかもね。

これは「ぜんぶぼくのせいだ」けどレポート頑張ってください。ちょとやそっとではOKは出ません。


昨日夕飯の時にスノーボードの大回転を観戦しました。

その時に話題になったのですが上村愛子さんに紫綬褒章が出ているんですね。ワールドカップチャンピョンだと出るようです。

学者で紫綬褒章といえばすごく高いレベル(例えばマッシー山本先生レベル)なのですが俳優だと三浦友和さんでも出るみたいですね。

ちょっと不公平な感じもします。

 


PDF
Tagged with:  

硫化水素研究者が一同に会する国際学会

the third international meeting on Hydrogen Sulfide Biology and its biomedical effects 

が今年の6月4日から6月6日京都で開催されます。

 

場所は京都大学医学部校内の芝蘭会館。

 

現在paperを募集中です

硫化水素関連としてはもっとも大きいこの会に皆さんも参加してみませんか?

 

会長は我らのキム兄こと国立精神・神経医療研究センター木村英雄先生

詳細はここからどうぞ。

 

もちろんぼくも参加します。

 

H2s main


PDF
Tagged with:  

一緒にやっていた田中先生の論文が”PLoS ONE”にアクセプトされました。ヤッホー!!
議論の書き直しなどで結構時間が掛かった様な気もしますが最終的には収まってよかったです。

この論文では結構重要な事を主張しているとぼくらは思っているのですが解らない人には解らないようです。

PLoS ONEですから一旦出版されれば無料で読むことができます。

General anesthetics inhibit erythropoietin induction under hypoxic conditions in the mouse brain

Background: Erythropoietin (EPO), originally identified as a hematopoietic growth factor produced in the kidney and fetal liver, is also endogenously expressed in the central nervous system (CNS). EPO in the CNS, mainly produced in astrocytes, is induced under hypoxic conditions in a hypoxia-inducible factor (HIF)-dependent manner and plays a dominant role in neuroprotection and neurogenesis. We investigated the effect of general anesthetics on EPO expression in the mouse brain and primary cultured astrocytes.

Methodology/Principal findings: BALB/c mice were exposed to 10% oxygen with isoflurane at various concentrations (0.10-1.0%). Expression of EPO mRNA in the brain was studied, and the effects of sevoflurane, halothane, nitrous oxide, pentobarbital, ketamine, and propofol were investigated. In addition, expression of HIF- 2α protein was studied by immunoblotting. Hypoxia-induced EPO mRNA expression in the brain was significantly suppressed by isoflurane in a concentration-dependent manner. A similar effect was confirmed for all other general anesthetics. Hypoxia- inducible expression of HIF-2α protein was also significantly suppressed with isoflurane. In the experiments using primary cultured astrocytes, isoflurane, pentobarbital, and ketamine suppressed hypoxia-inducible expression of HIF-2α protein and EPO mRNA.

Conclusions/Significance: Taken together, our results indicate that general anesthetics suppress activation of HIF-2 and inhibit hypoxia-induced EPO upregulation in the mouse brain through a direct effect on astrocytes.

このシリーズはこれに続いて最低二報出すつもりです。

カメラロール-325

少し前に”Antioxidants & Redox Signaling”に甲斐先生の論文が出たのですが紹介していませんでした。

Hydrogen Sulfide inhibits hypoxia- but not Anoxia-induced hypoxia-inducible factor 1 activation in a von Hippel-Lindau- and mitochondria-dependent manner

Antioxidants & Redox Signaling

Abstract

Aims: In addition to nitric oxide and carbon monoxide, hydrogen sulfide (H(2)S) is an endogenously synthesized gaseous molecule that acts as an important signaling molecule in the living body. Transcription factor hypoxia-inducible factor 1 (HIF-1) is known to respond to intracellular reduced oxygen (O(2)) availability, which is regulated by an elaborate balance between O(2) supply and demand. However, the effect of H(2)S on HIF-1 activity under hypoxic conditions is largely unknown in mammalian cells. In this study, we tried to elucidate the effect of H(2)S on hypoxia-induced HIF-1 activation adopting cultured cells and mice.
Results: The H(2)S donors sodium hydrosulfide and sodium sulfide in pharmacological concentrations reversibly reduced cellular O(2) consumption and inhibited hypoxia- but not anoxia-induced HIF-1α protein accumulation and expression of genes downstream of HIF-1 in established cell lines. H(2)S did not affect HIF-1 activation induced by the HIF-α hydroxylases inhibitors desferrioxamine or CoCl(2). Experimental evidence adopting von Hippel-Lindau (VHL)- or mitochondria-deficient cells indicated that H(2)S did not affect neosynthesis of HIF-1α protein but destabilized HIF-1α in a VHL- and mitochondria-dependent manner. We also demonstrate that exogenously administered H(2)S inhibited HIF-1-dependent gene expression in mice.

Innovations: For the first time, we show that H(2)S modulates intracellular O(2) homeostasis and regulates activation of HIF-1 and the subsequent gene expression induced by hypoxia by using an in vitro system with established cell lines and an in vivo system in mice. Conclusions: We demonstrate that H(2)S inhibits hypoxia-induced HIF-1 activation in a VHL- and mitochondria-dependent manner.

硫化水素の低酸素応答への影響を調べました。島根医大の竹永先生から供与していただいた細胞が大活躍でした。


PDF
Tagged with:  
madeonamac.gif Creative Commons License